3D细胞培养基质胶原蛋白
为什么要做3D 细胞培养?

传统的常规细胞培养由于细胞在体外改变的环境下增生逐渐丧失了原有的性状,往往和体内情况不相符;而动物实验完全在体内进行, 但由于体内的多种因素制约以及体内和外界环境相互影响而变得复杂化,难以研究单一过程,且难以研究中间过程。

 

3D 细胞培养是是以胶原水凝胶为细胞外基质支架,使细胞能够在载体的三维立体空间结构中迁移、生长。是介于单层细胞培养与动物实验之间的一种技术,既能最大程度的模拟体内环境,又能展现细胞培养的直观性及条件可控性的优势。

 

 

美国101Bio 新研发的3D 细胞培养基质胶——Col‐Tgel 具有目前市场上产品无法媲美的优势:

 

1.产品为纯的胶原,无其他杂质,如生长因子,激素等,产品稳定,批间差小,同时减少了干扰因素;

2.室温操作即可,无需在冰上进行;

3.耗时短,3D 培养仅需3 步完成,全程不超过1 个小时;

4.有3 种不同的硬度,可根据细胞样本选择最合适的硬度产品;

5.高透明度,便于观察;

6.2ml 包装,使用方便,无需反复冻融;

 

Product information


Cat.#

Stiffness

Volume

3 Stiffness Application

P720S-1

Soft

1 mL

Circulating cells, Nerve cells, BT474, HL60, P39

P720S-10

Soft

10 mL

P720M-1

Med

1 mL

Muscle cells, Adipose cells, C2C12, HepG2

P720M-10

Med

10 mL

P720H-1

Stiff

1 mL

Bone cells

P720H-10

Stiff

10 mL

P720SMH-3

S/M/H

1 mL x 3

A combo of P720S-1, P720M-1, P720H-1 for customer testing their cells.

   



Citations

1. KuwaharaKenrickZhi Yang, Ginger C. Slack, Marcel E. Nimni, and Bo Han. "Cell delivery using an injectable and adhesive transglutaminase–gelatin gel."Tissue Engineering Part C: Methods 16, no. 4 (2009): 609-618.


2. 
KuwaharaKenrick, Josephine Y. Fang, Zhi Yang, and Bo Han. "Enzymatic crosslinking and degradation of gelatin as a switch for bone morphogenetic protein-2 activity." Tissue Engineering Part A 17, no. 23-24 (2011): 2955-2964.


3. 
Fang, Josephine, Zhi Yang, ShihJye Tan, Charisse Tayag, Marcel E. Nimni, Mark Urata, and Bo Han. "Injectable gel graft for bone defect repair."Regenerative medicine 9, no. 1 (2014): 41-51.


4. 
Tan, ShihJye, Josephine Y. Fang, Zhi Yang, Marcel E. Nimni, and Bo Han. "The synergetic effect of hydrogel stiffness and growth factor on osteogenic differentiation." Biomaterials 35, no. 20 (2014): 5294-5306.


5. 
Fang, Josephine Y., Shih-Jye Tan, Zhi Yang, Charisse Tayag, and Bo Han. "Tumor Bioengineering Using a Transglutaminase Crosslinked Hydrogel." PloS one 9, no. 8 (2014): e105616.

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